A high-throughput screen for MscL channel activity and mutational phenotyping.
نویسندگان
چکیده
A novel fluorescence-based screen for bacterial mechanosensitive ion-channel activity has been developed. This assay is capable of clearly distinguishing the previously observed gain of function and loss of function phenotypes for the Escherichia coli mechanosensitive channel of large conductance (Ec-MscL). The method modifies Molecular Probes' Live/Dead BacLight bacterial viability assay to monitor MscL channel activity as a function of bacterial survival from osmotic downshock.
منابع مشابه
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عنوان ژورنال:
- Biochimica et biophysica acta
دوره 1514 2 شماره
صفحات -
تاریخ انتشار 2001